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ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. First, determine the number of WBC's from the hemocytometer as follows:
WBC count = (dilution ratio x # of cells counted x 10) / (# mm2 area counted) Then: WBC count = (20 x 100 x 10) / (8) = 2500 WBC/mm3 (or 2500 WBC/uL or 2.5 x 103 WBC/uL) Next, to find the WBC count per liter, multiply the WBC count/uL by the number of uL/L (there are 106 uL/L) So: (2.5 x 103 WBC/uL) x (106 uL/L) = 2.5 x 109 WBC/L Hematology A 1:20 dilution is made for a manual WBC count. The four corner squares on both sides of a hemocytometer are counted. A TOTAL of 100 cells are counted in that area. What is the white blood cell count in terms of a liter (? x 10^9/L)?

A) 250
B) 25000
C) 2.5
D) 2500
E) 25

2. The cells indicated by the arrows in the image are spherocytes. Spherocytes have a decreased surface to volume ratio and are therefore smaller with an increased MCHC. Spherocytes have lost their bi-concave shape and are spherical in shape, hence the name.
What are the cells that are indicated by the arrows in this peripheral blood smear image?

A) Echinocytes
B) Spherocytes
C) Stomatocytes
D) Elliptocytes

3. Bacterial contamination of platelets is most likely because they are stored at room temperature.
Bacterial contamination is MOST likely in which of the following blood products?

A) Platelets
B) Red Blood Cells
C) Fresh Frozen Plasma
D) Red Blood Cells Frozen

4. This is the total magnification of the high dry objective lens.

A) 100X
B) 400X
C) 40X
D) 1000X

5. The characteristic that distinguishes vancomycin-resistant Enterococcus faecium and Enterococcus faecalis from other Enterococcus species is their lack of motility.
Enterococci are all catalase-negative. Growth on bile esculin agar and in 6.5% salt broth are two characteristics that have commonly been used to identify Enterococcus to the genus level. A positive esculin in combination with a positive PYR reaction is another approach to presumptive identification.
Which one of these characteristics distinguishes vancomycin-resistant Enterococcus faecium and Enterococcus faecalis from other Enterococcus species?

A) Catalase negative
B) Non-motility
C) Growth on bile esculin agar
D) PYR positive reaction

Solutions: